Particle Dynamics in a Rotating Wall Vessel Bioreactor
نویسندگان
چکیده
Rotating bioreactors such as the High Aspect Ratio Vessel (HARV) provide a low shear and gentle mixing environment, ideal for mammalian cell culture in 3D. The HARV is a cylindrical “disc-shaped” batch culture vessel with no internal moving parts, that rotates about a single axis. Oxygenation is provided by a permeable silicon rubber membrane, allowing the diffusion of gases to and from the medium [1]. Cell culture in this bioreactor may be carried out using microcarriers and/or encapsulating cells within hydrogel particles. In our lab, tissue engineering from Embryonic Stem cells is of interest, and this has been implemented by inoculating the vessel with cells that have been previously encapsulated within Calcium alginate particles (Figure 1).
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